Biovit-12 Depot contains a long acting form of vitamin B₁₂ (Hydroxocobalamin), in addition to two important ingredients, namely folic acid and vitamin B₆. Vitamin B₁₂ is essential for growth, cell reproduction, hematopoiesis and myelin synthesis. It has its beneficial effects in vitamin B₁₂ deficiency anaemia and in increased vitamin B₁₂ requirements.

Folic acid is essential for normal erthropoiesis and is used in the management of megaloblastic aneamia in association with vitamin B₁₂.

Vitamin B₆ is required for amino acid metabolism. It is also involved in carbohydrate and lipid metabolism. Its deficiency affects mainly peripheral nerves and the hematopoietic system